Evaluation of the Effects of TIPTOPP’s Probiotics on Whiteleg Shrimp (Penaeus vannamei) Hatchery Production in a Controlled Environment
Direct application of TIPTOPP Pond combined with TIPTOPP AquaGut (T3) showed the most consistent positive effects: improved PL quality, higher stress resistance, and a tendency toward better survival. This method appears effective for reducing stress and improving production outcomes in hatcheries.
The fermentation approach (T2) with TIPTOPP products resulted in clearly lower performance than direct application. Based on this, we exclude fermentation for our bacteria and products. This results in earlier application, a simpler process, and higher ease of use in practical hatchery operations, while maintaining strong product performance.
Study Summary
This joint study aimed to evaluate the effects of TIPTOPP’s probiotics – TIPTOPP Pond (water bioremediation) and TIPTOPP AquaGut (in-feed probiotic) – on the production and quality of postlarvae (PL) of whiteleg shrimp (Penaeus vannamei).
Over a 22-day rearing period at ShrimpVet hatchery, three treatments were compared:
- T1 – Control: Standard hatchery practice without TIPTOPP products.
- T2 – Fermentation: Application of fermented TIPTOPP Pond according to protocol + TIPTOPP AquaGut in feed.
- T3 – Direct application: Daily direct dosing of TIPTOPP Pond + TIPTOPP AquaGut in feed.
Key Findings
- Best treatment vs. control:
- T3 (Direct application) clearly outperformed the control in several aspects: higher PL quality (muscle-to-gut ratio, average weight) and significantly higher stress resistance under salinity stress.
- PL10 survival in T3 was comparable to the control but with better quality parameters.
- This shows that direct application of TIPTOPP products maintains survival while improving robustness and uniformity of PLs.
- Water quality: Remained within optimal limits for larval rearing across all treatments.
- Bacteriological analysis: At certain periods, total bacterial counts in T2 and T3 were higher than the control, without negative effects. Vibrio remained within acceptable levels in all treatments.
- Stress resistance:
- Salinity stress: T3 significantly higher than T2, and better than control.
Formalin stress: T3 and control higher than T2.
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