Belgium

TipTopp Aquaculture – Proven bacteriostatic activity against AHPND/EMS Vibrio parahaemolyticus

Shrimp
Fish
Conclusion

This independent trial confirms the strong bacteriostatic potential of TipTopp probiotics against key shrimp pathogens, supporting their role as a sustainable and effective solution for AHPND/EMS prevention. Both TTA Pond and TTA Gut demonstrated broad activity, reinforcing their value in shrimp health management. These results form a solid scientific basis for TipTopp’s probiotic approach and pave the way for further in vivo validation in real-world farming conditions.

Trial Highlights

  • Objective: To evaluate the bacteriostatic activity of three proprietary TipTopp probiotic mixtures (TTA Pond, TTA Gut, CS606B) against major shrimp pathogens, including Vibrio parahaemolyticus (AHPND/EMS) and Vibrio campbellii.
  • Methods:
    • Agar Diffusion Suspension (ADS) – probiotic suspensions tested at different concentrations.
    • Agar Diffusion Colony Transfer (ADC) – freshly grown probiotic colonies placed directly on pathogen cultures.
  • Key positive findings:
    • TTA Pond and TTA Gut consistently inhibited multiple Vibrio pathogens, showing both clear and slight inhibition across most strains.
    • CS606B showed targeted inhibition against V. parahaemolyticus TW01.
    • Colony transfer tests demonstrated broad-spectrum activity for TTA Pond and TTA Gut, confirming strong probiotic-pathogen interaction.
    • Even at high challenge levels, TipTopp products demonstrated measurable antibacterial effects.
  • Insight: The method of probiotic preparation (liquid vs. solid culture) influences inhibition levels, offering opportunities to optimise field application for maximum pathogen control.
Get full access to the research
Leave your contact details to receive the complete study directly in your inbox.
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.

Proven in practice
recent insights

EVALUATION THE EFFECTS OF TIPTOPP'S PRODUCTS ON GROWTH PERFORMANCE OF Penaeus monodon POSTLARVAE IN DIFFERENT NURSERY PROTOCOLS
Read summary
The effect of TIPTOPP's TipTopp Gut and Pond products in mitigating Acute Hepatopancreatic Necrosis Disease (AHPND) in whiteleg shrimp (Penaeus vannamei) under laboratory conditions
Read summary
Feeding Trial: Effects of TipTopp Pond and TipTopp Gut Probiotics on Growth Performance, Survival, and Water Quality in Whiteleg Shrimp (Penaeus vannamei) under Controlled Laboratory Conditions
Read summary

Trusted by farmers
Leading Aquaculture Farms

Read case
TipTopp Success Story – TEPBAC, Vietnam
“After just two weeks using TipTopp Gut, our shrimp’s gut health became visibly healthier and more stable, Vibrio levels dropped, and our pond liners were cleaner. The product is efficient, easy to use, and does exactly what it promises.”
No items found.
Read case
TipTopp Success Story – Calica Camaronera del Litoral, Ecuador
“With TipTopp Pond we improved oxygen levels, reduced organic matter, and achieved better feed conversion ratios. It is easy to use, saves labor time, and most importantly, it has boosted survival and growth in our ponds.”
No items found.

Lets talk
about opportunities

Want to see what TippTopp can do for your farm? Book a free consultation with our team, no strings attached.
More than 1,000 farmers are already reaping the benefits of probiotics.
TipTopp benefits
Easy to use product in every phase of aquatic cultivation
Higher yield
High quality and consistent products
TipTopp benefits
Assistance with implementation.
We offer production capacity.
Science-based
Do you have any questions? info@tiptoppaquaculture.com
Europe
Belgium
Laboratory disease trial
Shrimp